Journal: Stroke

Article Title: Dihydrolipoic acid inhibits lysosomal rupture and NLRP3 through LAMP1/CaMKII/TAK1 pathways after subarachnoid hemorrhage in rat

doi: 10.1161/STROKEAHA.117.018593

Figure Lengend Snippet: (A) Modified Garcia score. (B) Beam balance score. (C) Representative Western blot images and quantitative analyses of LAMP1, p-CaMKIIα, p-TAK1, p-JNK, NLRP3, cleaved caspase-1and IL-1β. n=6 per group. *P < 0.05, **P < 0.01 vs. Sham group; #P < 0.05, ##P < 0.01 vs. SAH+vehicle group. & P < 0.05, && P < 0.01 vs. SAH+DHLA+Scr siRNA group. Scr, scramble. siRNA, small interfering ribonucleic acid Bars represent mean ± SEM.

Article Snippet: LAMP1 siRNA (SR508640), CaMKIIα siRNA (SR500835) and scramble siRNA were purchased from Origene Technologies, Inc. (MD, USA).

Techniques: Modification, Western Blot

Journal: Stroke

Article Title: Dihydrolipoic acid inhibits lysosomal rupture and NLRP3 through LAMP1/CaMKII/TAK1 pathways after subarachnoid hemorrhage in rat

doi: 10.1161/STROKEAHA.117.018593

Figure Lengend Snippet: (A) Modified Garcia score. (B) Beam balance score. (C) Representative Western blot bands and quantitative analyses of p-CaMKIIα, p-TAK1, p-JNK, NLRP3. n=6 per group. **P < 0.01 vs. Sham group; #P < 0.05, ##P < 0.01 vs. SAH+Scr siRNA group. Bars represent mean ± SEM.

Article Snippet: LAMP1 siRNA (SR508640), CaMKIIα siRNA (SR500835) and scramble siRNA were purchased from Origene Technologies, Inc. (MD, USA).

Techniques: Modification, Western Blot

Journal: Autophagy

Article Title: WIPI1 promotes fission of endosomal transport carriers and formation of autophagosomes through distinct mechanisms

doi: 10.1080/15548627.2021.1886830

Figure Lengend Snippet: Antibodies used

Article Snippet: LAMP1 , H4A3 , H4A3 , USBiological Life Sciences , 1/500; 1/1000 , IF WB.

Techniques: Transduction

Journal: AMB Express

Article Title: Preparation of a camel-canine chimeric antibody against canine B-cell lymphoma

doi: 10.1186/s13568-025-01974-7

Figure Lengend Snippet: Immunofluorescence analysis indicated that the anti-cCD20 sdAb binds to CD20 on Raji cells. Raji cells were fixed on coverslips and subjected to immunofluorescence staining. The anti-cCD20 sdAb served as the primary antibody, and CoraLite ® 488-conjugated 6×His-Tag mouse monoclonal antibody, diluted 1:250, was used as the secondary antibody, with PBS as the blank control. The fluorescence signals were detected using a confocal microscope (Zeiss, LSM-800). The cells were observed to be in good condition under a bright field ( a ), bright green fluorescence was observed in the anti-cCD20 sdAb-stained cells ( b ), whereas no fluorescence was detected in the control group ( c )

Article Snippet: After the cultured Raji cells were fixed, the anti-cCD20 sdAb served as the primary antibody, and a CoraLite ® 488-conjugated 6×His His-Tag mouse monoclonal antibody was the secondary antibody.

Techniques: Immunofluorescence, Staining, Control, Fluorescence, Microscopy